Scientists reveal characteristics of Salmonella dry surface biofilm

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Food scientists at the National University of Singapore (NUS) have revealed key characteristics of the foodborne pathogen Salmonella dry surface biofilm (DSB), a previously overlooked type of biofilm that commonly exists in dry food processing environments.

Bacterial biofilm formation in the food industry is a significant concern, affecting food safety, production efficiency, economic loss, and consumer trust. Most existing research on foodborne pathogenic biofilms has focused on wet surface biofilms (WSBs), as it is generally believed that microorganisms cannot survive under dry conditions, let alone form biofilms.

However, recent recalls and outbreaks of Salmonella associated with low-moisture food (LMF) have provided mounting evidence that pathogens can persist in dry conditions.

A research team led by Assistant Professor LI Dan from the NUS Department of Food Science and Technology has provided a detailed characterization of the Salmonella DSBs examining their morphology, single-cell landscape, and response to sanitization techniques in comparison to traditional WSBs. The efforts of this research offer valuable insights for managing dry biofilms in the food industry.

The research findings were published in the journal Applied and Environmental Microbiology on 4 November 2024.

The study explored the structural and functional characteristics of Salmonella Typhimurium DSB using advanced microscopy techniques, including confocal laser scanning, transmission electron, and scanning electron microscopy.

These analyses revealed unique features of DSB compared to WSB. Specifically, DSB cells are surrounded by a dense, compact capsule that likely contributes to their higher antimicrobial resistance, while WSB cells display distinct plasma and outer membrane layers. Additionally, DSBs exhibit a "sandwich-like" vertical structure, with intact cells at the core and damaged cells on the outer layer.

To understand functional diversity within the biofilm, single-cell transcriptomics was employed, identifying bacterial clusters with active antioxidative and virulence functions—highlighting potential safety concerns for LMFs. The team also developed a water-free antibiofilm strategy tailored for the LMF industry. This method combines the flavonoid morin with 70% isopropyl alcohol, offering an effective sanitization method without requiring water.

Asst Prof Li said, "The outcomes of this research are of critical importance, offering several pivotal insights. By detailing the structural and functional properties of Salmonella DSB, we illuminate how these biofilms resist desiccation and sanitization, which are prevalent challenges in LMF environments.

"The application of single-cell RNA sequencing allows us to uncover the heterogeneity within DSB populations. These findings challenge the perception of dehydrated biofilm cells as uniformly dormant and underscore the persistent threat posed by these pathogens.

"With the advent of flavonoid-based sanitizers, the food industry can adopt more effective, eco-friendly, and scalable solutions for biofilm management. Implementing such strategies could significantly reduce contamination events, enhance consumer trust, and minimize financial losses associated with recalls and outbreaks.

"Taken together, these outcomes reaffirm our commitment to translating scientific discoveries into actionable solutions for food safety and public health."

More information: Zejia Lin et al, Salmonella dry surface biofilm: morphology, single-cell landscape, and sanitization, Applied and Environmental Microbiology (2024). DOI: 10.1128/aem.01623-24

Journal information: Applied and Environmental Microbiology

Provided by National University of Singapore